Working with Antibiotics

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Antibiotic Information

Antibiotic Abbreviation Stock Solution

Concentration

Solvent Stock Concentration to

Working Concentration

Notes
Ampicillin Amp 100mg/ml H2O 1000x Plates only last 2 weeks
Carbenicillin Crb 100mg/ml 50% Ethanol 1000x
Chloramphenicol Cam 20mg/ml 100% Ethanol 1000x
Cycloheximide Chx 100mg/ml DMSO 1000x Toxic to fungi and animals (including humans)

Use protective gear; work in chemical hood.

Erythromycin Ery 250mg/ml 100% Ethanol 1000x
Kanamycin Kan 50mg/ml H2O 1000x Plates only last 2 weeks
Spectinomycin Spec 60mg/ml H2O 1000x
Streptomycin Str 100mg/ml H2O 1000x
Tetracycline Tet 10mg/ml 70% Ethanol 1000x Light sensitive

Wrap tubes and plates in aluminum foil


Preparing Antibiotics

Prepare 10mL of antibiotics at a time (less, for antibiotics that degrade quickly, like Ampicillin, Kanamycin, and Tetracycline).

If the solvent is not water, we can assume that the antibiotics will be sterilized by the solvent. After creating the appropriate concentration, they should be aliquoted into sterile microcentrifuge tubes by 500µl and put in the -20 freezer.

If the solvent is in water, prepare the solution in a 50ml conical tube. Filter sterilize the solution through a 0.22µm filter and a 10mL syringe, all in the biological hood using sterile technique. Aliquote into microcentrifuge tubes by 500µl and put in the -20 freezer

Be sure to label the aliquots with the antibiotic, concentration, and date. Please wrap Tetracycline aliquots in foil.