Working with Antibiotics
|Solvent||Stock Concentration to
|Ampicillin||Amp||100mg/ml||H2O||1000x||Plates only last 2 weeks|
|Cycloheximide||Chx||100mg/ml||DMSO||1000x||Toxic to fungi and animals (including humans)
Use protective gear; work in chemical hood.
|Kanamycin||Kan||50mg/ml||H2O||1000x||Plates only last 2 weeks|
|Tetracycline||Tet||10mg/ml||70% Ethanol||1000x||Light sensitive
Wrap tubes and plates in aluminum foil
Prepare 10mL of antibiotics at a time (less, for antibiotics that degrade quickly, like Ampicillin, Kanamycin, and Tetracycline).
If the solvent is not water, we can assume that the antibiotics will be sterilized by the solvent. After creating the appropriate concentration, they should be aliquoted into sterile microcentrifuge tubes by 500µl and put in the -20 freezer.
If the solvent is in water, prepare the solution in a 50ml conical tube. Filter sterilize the solution through a 0.22µm filter and a 10mL syringe, all in the biological hood using sterile technique. Aliquote into microcentrifuge tubes by 500µl and put in the -20 freezer
Be sure to label the aliquots with the antibiotic, concentration, and date. Please wrap Tetracycline aliquots in foil.