Reagent Recipes

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Reagent Information

Reagent Abbreviation Stock Solution

Concentration

Solvent Stock Concentration to

Working Concentration

Notes
Bromo-chloro-indolyl-galactopyranoside X-gal 40mg/ml Dimethylformamide (DMF) 500x Use DMF in a chemical hood
Isopropyl β-D-1-thiogalactopyranoside IPTG 0.1M H2O 1000x Light sensitive. Molecular weight: 238.31 g/mol


Glycerol for freezing aliquots

We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%.

10x PBS

Used to create 1x PBS for dilution or washing cells of any species.

For 1 liter:

    • NaCl 80g
    • KCl 2g
    • Na2HPO4 14.4g
    • KH2PO4 2.4g

Stir salts with MilliQ water. This may take a significant time to dissolve!

Adjust pH with NaOH to 7.4. This will take a significant amount of NaOH; add in 200ul increments.