Generic PCR

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Generic PCR Recipe

For a 10ul reaction:

Reagent Volume
OneTaq Reaction Buffer (5X) 2ul
dNTPs (Nucleotides; 40 mM total) XXul
Forward Primer (10uM) 0.75ul
Reverse Primer (10uM) 0.75ul
DNA template (< 50ng/ul) 1.0ul
OneTaq Polymerase (XXU/ul) 0.1ul
Molecular-grade Water 5.Xul

Generic PCR Recipe with DMSO

1% DMSO (0.1ul in 10ul reaction)